Isaac Arnquist

 

Personal Information

I am a second year graduate student in the Holcombe Group.

I was born on the plains of western Nebraska, but grew up among the many lakes and Scandinavians of Minnesota.  I graduated from St. Olaf College in Northfield, Minnesota with a B.A. in chemistry in the spring of 2005.  I spent the next two years teaching English in Japan while trying to eat as much sushi and have as many adventures as possible.  I was recently married to my wife Enid, who I met at a sumo tournament in Japan (no, she is not a sumo wrestler).  I enjoy camping, hiking, brewing beer, growing a beard once a year, playing Scrabble, and using Oxford commas.

Isaac

Current Research

Haley and I are busy working on a metallomics project in which we are trying to determine two things:  (1) qualitative information about which metal(s) is associated with each protein within a cell, and (2) the formation constant value (Kf) between the protein and the metal.  We are using one dimensional polyacrylamide gel electrophoresis coupled offline with laser ablation inductively coupled plasma mass spectrometry, or more endearingly known as 1-D PAGE LA-ICPMS.  We may also utilize the ETV in these studies. 

 

Undergraduate Research

My undergraduate research focused on designing and optimizing undergraduate lab experiments for a new bioanalytical chemistry class being offered at St. Olaf College.  Most of the experiments involved determining peptides and proteins using a variety of methods, like 1- and 2-D gel electrophoresis, size-exclusion chromatography, LC-MS, and others.  In particular, however, most of my research revolved around the use of ESI-MS in the development of novel proteomic experiments.  Three of these experiments have been published:

  • Arnquist, Isaac J.; Beussman, Douglas J.  Incorporating biological mass spectrometry into undergraduate teaching labs, Part 1: Identifying proteins based on molecular mass.  Journal of Chemical Education  (2007),  84(12),  1971-1973.
  • Arnquist, Isaac J.; Beussman, Douglas J.  Incorporating biological mass spectrometry into undergraduate teaching labs, Part 2: Peptide identification via isotopic patterns.  Journal of Chemical Education (Accepted for Publication).
  • Arnquist, Isaac J.; Beussman, Douglas J.  Incorporating biological mass spectrometry into undergraduate teaching labs, Part 3: De novo peptide sequencing using electrospray tandem mass spectrometry.  Journal of Chemical Education (Accepted for Publication).


Contact Information

The University of Texas at Austin
Department of Chemistry and Biochemistry
1 University Station A5300
Austin, TX 78712-0165

Phone (512) 471-1180
Fax (512) 471-0985

isaac.arnquist@mail.utexas.edu